anti fading mounting medium with dapi  (Beijing Solarbio Science)

Journal: NPJ Vaccines

Article Title: Oral delivery of Trichinella spiralis antigens by yeast-derived β-glucan particles enhances anti-helminth immunity

doi: 10.1038/s41541-026-01458-0

Figure Lengend Snippet: A CLSM images showing RB-Ag-GPs (red) internalized by PKH67-stained (green) Raw264.7, DC2.4, and Caco-2 cells with DAPI-labeled nuclei (blue) after incubation. B Ex vivo fluorescence imaging of BALB/c mice orally administered PBS or FITC-Ag-GPs, showing biodistribution in secondary lymphoid tissues (ILN, PP, MLN), IE, and major organs (heart, liver, spleen, lung, kidney, small intestine), with fluorescence intensity indicating Ag-GPs uptake. C – F Flow cytometry analysis of BMDCs activation. C Single-cell gating; D CD11c⁺ DC identification; E CD86⁺CD80⁺ double-positive cell selection; F Quantification of activated DCs after treatment with PBS, Ag, GPs, or Ag-GPs. Data are presented as mean ± SD ( n = 3), **** P < 0.0001.

Article Snippet: Raw264.7, DC2.4, and Caco-2 cells in 24-well plates were incubated with RB-Ag-GPs for 4 h, fixed with 4% paraformaldehyde, stained with PKH67 green fluorescent cell linker kit (PKH67GL, Sigma-Aldrich, Germany) for membrane labeling and DAPI anti-fade mounting medium (DAPI, HY-K1047, MedChemExpress, USA) for nuclear staining, followed visualized by CLSM.

Techniques: Staining, Labeling, Incubation, Ex Vivo, Fluorescence, Imaging, Flow Cytometry, Activation Assay, Single Cell, Selection

Journal: NPJ Vaccines

Article Title: Oral delivery of Trichinella spiralis antigens by yeast-derived β-glucan particles enhances anti-helminth immunity

doi: 10.1038/s41541-026-01458-0

Figure Lengend Snippet: A CLSM images of RB-Ag-GPs (red) uptake in Raw264.7 and DC2.4 cells pre-treated with PBS or LA (10 μg/mL, 2 h). Nuclei counterstained with DAPI (blue). B Ex vivo fluorescence imaging of FITC-Ag-GPs distribution in LA or PBS-pretreated mice at 4 h post-administration. Tissues analyzed: ILN, PP, MLN, IE, and major organs. C Dectin-1 mRNA expression levels in intestinal tissues of immunized mice at 7 dpi. Data are presented as mean ± SD ( n = 3). ns not significant, *** P < 0.01, **** P < 0.0001. D , E Larvae per gram (LPG) of muscle burden and the reduction rate in mice following T. spiralis infection with vaccination at 35 dpi. Data are presented as mean ± SD ( n = 3), * P < 0.05, ** P < 0.01. F – H Ag-specific serum antibody levels (IgG, IgE, SIgA) in orally immunized mice. Data are presented as mean ± SD ( n = 3), * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: Raw264.7, DC2.4, and Caco-2 cells in 24-well plates were incubated with RB-Ag-GPs for 4 h, fixed with 4% paraformaldehyde, stained with PKH67 green fluorescent cell linker kit (PKH67GL, Sigma-Aldrich, Germany) for membrane labeling and DAPI anti-fade mounting medium (DAPI, HY-K1047, MedChemExpress, USA) for nuclear staining, followed visualized by CLSM.

Techniques: Ex Vivo, Fluorescence, Imaging, Expressing, Infection

Journal: Materials Today Bio

Article Title: 4D fabrication of scaffolds facilitates the construction of cholangiocyte monolayers from human and mouse liver derived organoids

doi: 10.1016/j.mtbio.2025.102757

Figure Lengend Snippet: PT-PLATMC@PDA scaffold supports the survival, proliferation and spreading of cholangiocytes to form a monolayer. Cell viability (A) and density (B) of cholangiocytes seeded on PT-PLATMC@PDA films in culture for 1 day, 3 days, and 7 days (n = 3). Fluorescence microscope images of mouse liver derived cholangiocyte organoid seeded on the PT-PLATMC@PDA film in culture for 7 days (C), 14 days (D), 21 days (E) and 35 days (F) stained with DAPI, ZO-1, and CK19. DAPI and ECAD staining of the 21-day cultured mouse cholangiocytes (G) and 37-day cultured human cholangiocytes (H) seeded SMP PT-PLATMC@PDA planar films.

Article Snippet: The samples were washed with PBS, mounted with 100 μl of anti-fade mounting medium with DAPI (Vector Laboratories Inc, USA), and analyzed under a confocal laser scanning microscope (Zeiss LSM880 with Ariyscan, Germany).

Techniques: Fluorescence, Microscopy, Derivative Assay, Staining, Cell Culture

Journal: Materials Today Bio

Article Title: 4D fabrication of scaffolds facilitates the construction of cholangiocyte monolayers from human and mouse liver derived organoids

doi: 10.1016/j.mtbio.2025.102757

Figure Lengend Snippet: Construction of a single-layer bile duct epithelium on PT-PLATMC@PDA tubular scaffolds. PT-PLATMC@PDA tubes seeded with (A) mouse derived cholangiocyte organoids cultured for 21 days and (B) human derived cholangiocyte organoids cultured for 37 days immuno-stained for DAPI, ZO-1and/or F Actin.

Article Snippet: The samples were washed with PBS, mounted with 100 μl of anti-fade mounting medium with DAPI (Vector Laboratories Inc, USA), and analyzed under a confocal laser scanning microscope (Zeiss LSM880 with Ariyscan, Germany).

Techniques: Derivative Assay, Cell Culture, Staining

Journal: bioRxiv

Article Title: Sex-specific differences in endocannabinoid regulation of cocaine-evoked dopamine in the medial nucleus accumbens shell

doi: 10.64898/2026.03.27.714857

Figure Lengend Snippet: Experimental design for in vivo fiber photometry recordings with pharmacological testing. (A) Viral approach and implantation targeting for the NAc ms . (B) Representative histology of viral expression and fiber optic cannula targeting. DAPI signal is blue and dLight 1.3b is green. (C) dLight 1.3b expression in the NAc ms . Viral expression is prominent around the fiber optic cannula tip, which is situated in the more dorsal aspect of the NAc ms . (D) Fiber optic cannula tip placement for all rats included in the study (anterior/posterior distance from bregma ) (E) Visual representation of behavioral design for fiber photometry experiments. Rats can be pretreated with endocannabinoid-modulating drugs or their corresponding vehicle and tested for photometry signal changes after an experimenter-delivered intravenous infusion of cocaine or saline. (F) Overview of experimental testing regimen. Rats generally completed two or more experimental schedules. For pharmacological testing, vehicle pretreatment always preceded Rimonabant or MJN-110 pretreatment, and the cocaine dose remained consistent for each test day. For cocaine dose response testing, rats received escalating doses of cocaine, starting with a saline control.

Article Snippet: The next day, slices were washed with PBS at room temperature and then mounted on microscope slides and coverslipped with Vectashield anti-fade mounting medium with DAPI (Vector Laboratories, Plain City, OH).

Techniques: In Vivo, Expressing, Saline, Control